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monarch spin rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch spin rna cleanup kit
    Monarch Spin Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monarch+rna+cleanup+kit/Monarch+Spin+RNA+Cleanup+Kit/pmc13620113-224-17-22
    Average 99 stars, based on 1534 article reviews
    monarch spin rna cleanup kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Universal conditional knockout approach to multiple species, from fish to human induced pluripotent stem cells
    Article Snippet: .. The pCS2-Cre.zf1 (Addgene plasmid #61 391) [ ] plasmid DNA was linearized by KpnI and synthesized using the mMESSAGE mMACHINE SP6 Transcription Kit (Cat # AM1340, Thermo Fisher Scientific), and purified with Monarch ® RNA Cleanup Kit (Cat # T2040L, New England BioLabs), according to the manufacturer’s instructions. ..

    Synthesized:

    Article Title: Universal conditional knockout approach to multiple species, from fish to human induced pluripotent stem cells
    Article Snippet: .. The pCS2-Cre.zf1 (Addgene plasmid #61 391) [ ] plasmid DNA was linearized by KpnI and synthesized using the mMESSAGE mMACHINE SP6 Transcription Kit (Cat # AM1340, Thermo Fisher Scientific), and purified with Monarch ® RNA Cleanup Kit (Cat # T2040L, New England BioLabs), according to the manufacturer’s instructions. ..


    Article Title: Modular mRNA cocktail enables synergistic activation of antigen-specific T cells for cancer immunotherapy
    Article Snippet: .. The synthesized mRNA was subsequently purified with the Monarch RNA Cleanup Kit (New England Biolabs), and its integrity was assessed using an RNA ScreenTape assay (Agilent Technologies). .. mRNA transfection in vitro mRNA transfection was performed using the TransIT-mRNA Transfection Kit (Mirus Bio), a cationic lipid-based reagent.

    Purification:

    Article Title: Universal conditional knockout approach to multiple species, from fish to human induced pluripotent stem cells
    Article Snippet: .. The pCS2-Cre.zf1 (Addgene plasmid #61 391) [ ] plasmid DNA was linearized by KpnI and synthesized using the mMESSAGE mMACHINE SP6 Transcription Kit (Cat # AM1340, Thermo Fisher Scientific), and purified with Monarch ® RNA Cleanup Kit (Cat # T2040L, New England BioLabs), according to the manufacturer’s instructions. ..

    Article Title: Computational design of optimized and preferentially paired human TCR constant regions for improved T cell function
    Article Snippet: .. In vitro–transcribed mRNA for both TCR chains was generated using HighYield T7 ARCA mRNA Synthesis Kit (Jena Bioscience, Jena, Germany) and purified using Monarch RNA Cleanup Kit (T2040L, New England Biolabs). ..

    Article Title: Rv3787c Influences Growth Dynamics of Mycobacterium tuberculosis .
    Article Snippet: .. The RNA samples were further purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions and quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. Residual genomic DNA was removed by treating 2 μg of RNA from each sample with DNase I (Ambion, Life Technologies Invitrogen, CA, USA).


    Article Title: Modular mRNA cocktail enables synergistic activation of antigen-specific T cells for cancer immunotherapy
    Article Snippet: .. The synthesized mRNA was subsequently purified with the Monarch RNA Cleanup Kit (New England Biolabs), and its integrity was assessed using an RNA ScreenTape assay (Agilent Technologies). .. mRNA transfection in vitro mRNA transfection was performed using the TransIT-mRNA Transfection Kit (Mirus Bio), a cationic lipid-based reagent.

    Article Title: Barcoded Sleeping Beauty Transposons as Mobile Reporters of Chromatin Architecture
    Article Snippet: Barcode-containing RNA was generated by overnight in vitro transcription using the Vazyme T7 High Yield RNA Transcription Kit (TR101) according to the manufacturer’s instructions. .. RNA was purified using the NEB Monarch RNA Cleanup Kit, and concentration was measured prior to use; 300–1000 ng of RNA was taken for downstream processing. .. Reverse transcription was performed using the Vazyme HiScript III 1st Strand cDNA Synthesis Kit with gDNA Wiper (R312) according to the manufacturer’s instructions, with 0.5 μL of 100 μM RT primer p6 (5′-TCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNN-3′).

    Article Title: Delivery of monoclonal antibodies using mRNA lipid nanoparticles confers protection against SARS-CoV-2 and influenza
    Article Snippet: The DNA was then PCR amplified and used as a template for in vitro transcription of mRNA using T7 RNA polymerase (New England Biolab), with all uridine residues substituted by N1-methylpseudouridine (BOC Sciences). .. The mRNA products were purified using a Monarch RNA Cleanup Kit (NEB), eluted in 1 mM sodium citrate, and sterile-filtered with a 0.22-μm syringe filter. ..

    Generated:

    Article Title: Computational design of optimized and preferentially paired human TCR constant regions for improved T cell function
    Article Snippet: .. In vitro–transcribed mRNA for both TCR chains was generated using HighYield T7 ARCA mRNA Synthesis Kit (Jena Bioscience, Jena, Germany) and purified using Monarch RNA Cleanup Kit (T2040L, New England Biolabs). ..

    Spectrophotometry:

    Article Title: Rv3787c Influences Growth Dynamics of Mycobacterium tuberculosis .
    Article Snippet: .. The RNA samples were further purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions and quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). .. Residual genomic DNA was removed by treating 2 μg of RNA from each sample with DNase I (Ambion, Life Technologies Invitrogen, CA, USA).

    Concentration Assay:

    Article Title: Barcoded Sleeping Beauty Transposons as Mobile Reporters of Chromatin Architecture
    Article Snippet: Barcode-containing RNA was generated by overnight in vitro transcription using the Vazyme T7 High Yield RNA Transcription Kit (TR101) according to the manufacturer’s instructions. .. RNA was purified using the NEB Monarch RNA Cleanup Kit, and concentration was measured prior to use; 300–1000 ng of RNA was taken for downstream processing. .. Reverse transcription was performed using the Vazyme HiScript III 1st Strand cDNA Synthesis Kit with gDNA Wiper (R312) according to the manufacturer’s instructions, with 0.5 μL of 100 μM RT primer p6 (5′-TCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNN-3′).

    Sterility:

    Article Title: Delivery of monoclonal antibodies using mRNA lipid nanoparticles confers protection against SARS-CoV-2 and influenza
    Article Snippet: The DNA was then PCR amplified and used as a template for in vitro transcription of mRNA using T7 RNA polymerase (New England Biolab), with all uridine residues substituted by N1-methylpseudouridine (BOC Sciences). .. The mRNA products were purified using a Monarch RNA Cleanup Kit (NEB), eluted in 1 mM sodium citrate, and sterile-filtered with a 0.22-μm syringe filter. ..

    Isolation:

    Article Title: A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase center
    Article Snippet: Subsequently, 0.15 U/ml Turbo DNase (Invitrogen, Cat No. AM2238) was added, and the reaction was incubated at 37 °C for 30 min to digest the plasmid DNA. .. The transcribed mRNA was isolated using the Monarch RNA Cleanup Kit (New England Biolabs, Cat No. T2040 L), eluted in 1 mM sodium citrate pH 6.5 (Invitrogen, Cat. No. AM7001), and quantified by A260 measurement. .. The p200-RLuc reporter was capped using the Vaccinia Capping System (NEB, Cat. No. M2080S) according to the manufacturer’s instructions, with the modification that 1 U/ml RNase inhibitor (Vazyme, Cat. No. R301–03) was added to the reaction.



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    MRT and MRT-CBD construct design, purification, storability, and optimal enzyme-to-template ratio. (A) Schematic representation (not to scale) of MRT and updated MRT-CBD construct in a new plasmid backbone with a C-terminal CBD domain. (B) Overview of the optimized one-step protein production workflow. (C) Representative 10% SDS-PAGE gel (1.5 mm) of MRT and MRT-CBD expression. in – pooled elution fractions from affinity purification; conc – concentrated protein. Bands representing full-length protein are indicated (dot = MRT, triangle = MRT-CBD), colours correspond to panel A. Marker: PageRuler broad range unstained protein ladder. (D) Representative Western blot of concentrated protein detected by anti-His antibody. Full-length protein indicated by dot (MRT) or triangle (MRT-CBD). Densitometry analysis of the full-length protein is shown next to the plot, full-length protein band marked by blue dashed line. (E, F) Protein storability. Top panels: activity of samples stored at −20°C; bottom panels: activity of samples stored at −70°C. Measurements were performed in technical duplicates, except the first time point ( n = 3). Black outline around shapes indicates the use of a new <t>RNA</t> template batch. (E) protein activity over 52 weeks. (F) Activity during repeated freeze-thaw cycles over 20 weeks. (G) Bar plot of densitometry analysis of free cDNA produced by indicated enzyme amount (U) from 100 ng of 3’-adapter-ligated tRNA template. 0.5 units of the same MRT-CBD reaction were loaded on both gels for comparison.
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    Image Search Results


    MRT and MRT-CBD construct design, purification, storability, and optimal enzyme-to-template ratio. (A) Schematic representation (not to scale) of MRT and updated MRT-CBD construct in a new plasmid backbone with a C-terminal CBD domain. (B) Overview of the optimized one-step protein production workflow. (C) Representative 10% SDS-PAGE gel (1.5 mm) of MRT and MRT-CBD expression. in – pooled elution fractions from affinity purification; conc – concentrated protein. Bands representing full-length protein are indicated (dot = MRT, triangle = MRT-CBD), colours correspond to panel A. Marker: PageRuler broad range unstained protein ladder. (D) Representative Western blot of concentrated protein detected by anti-His antibody. Full-length protein indicated by dot (MRT) or triangle (MRT-CBD). Densitometry analysis of the full-length protein is shown next to the plot, full-length protein band marked by blue dashed line. (E, F) Protein storability. Top panels: activity of samples stored at −20°C; bottom panels: activity of samples stored at −70°C. Measurements were performed in technical duplicates, except the first time point ( n = 3). Black outline around shapes indicates the use of a new RNA template batch. (E) protein activity over 52 weeks. (F) Activity during repeated freeze-thaw cycles over 20 weeks. (G) Bar plot of densitometry analysis of free cDNA produced by indicated enzyme amount (U) from 100 ng of 3’-adapter-ligated tRNA template. 0.5 units of the same MRT-CBD reaction were loaded on both gels for comparison.

    Journal: RNA Biology

    Article Title: Production of MarathonRT and its comparison with commercial reverse transcriptases for tRNA sequencing library preparation

    doi: 10.1080/15476286.2026.2720028

    Figure Lengend Snippet: MRT and MRT-CBD construct design, purification, storability, and optimal enzyme-to-template ratio. (A) Schematic representation (not to scale) of MRT and updated MRT-CBD construct in a new plasmid backbone with a C-terminal CBD domain. (B) Overview of the optimized one-step protein production workflow. (C) Representative 10% SDS-PAGE gel (1.5 mm) of MRT and MRT-CBD expression. in – pooled elution fractions from affinity purification; conc – concentrated protein. Bands representing full-length protein are indicated (dot = MRT, triangle = MRT-CBD), colours correspond to panel A. Marker: PageRuler broad range unstained protein ladder. (D) Representative Western blot of concentrated protein detected by anti-His antibody. Full-length protein indicated by dot (MRT) or triangle (MRT-CBD). Densitometry analysis of the full-length protein is shown next to the plot, full-length protein band marked by blue dashed line. (E, F) Protein storability. Top panels: activity of samples stored at −20°C; bottom panels: activity of samples stored at −70°C. Measurements were performed in technical duplicates, except the first time point ( n = 3). Black outline around shapes indicates the use of a new RNA template batch. (E) protein activity over 52 weeks. (F) Activity during repeated freeze-thaw cycles over 20 weeks. (G) Bar plot of densitometry analysis of free cDNA produced by indicated enzyme amount (U) from 100 ng of 3’-adapter-ligated tRNA template. 0.5 units of the same MRT-CBD reaction were loaded on both gels for comparison.

    Article Snippet: Bands corresponding to 5.8S and 5S rRNA are indicated. (B) binding capacity of Monarch RNA Cleanup columns (10 μg, NEB) loaded with 10, 5, or 2.5 μg total RNA.

    Techniques: Construct, Purification, Plasmid Preparation, SDS Page, Expressing, Affinity Purification, Marker, Western Blot, Analysis, Activity Assay, Produced, Comparison

    tRNA column and gel extraction for sequencing. (A) schematic overview of the tRNA silica-based spin column purification process. First, the total RNA sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. DA/DPH denotes deacylated and dephosphorylated total RNA and encircled roman numerals I and II correspond to eluates from column I and II, respectively. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low‑range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) binding capacity of Monarch RNA Cleanup columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. input – total RNA post-phenol/BCP extraction; DA/DPH – deacylated and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) urea-PAA analysis of eluate from column I and II. Note removal of rRNA in eluate II. Equal volumes were loaded of both column eluates. (D) column enriched and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples ( n = 48). Encircled areas correspond to column enriched tRNA (green ellipse) or gel-extracted tRNA (black ellipse). (F) correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n = 5, gel extraction n = 3). Pearson’s r = 0.9998, R 2 = 0.9995.

    Journal: RNA Biology

    Article Title: Production of MarathonRT and its comparison with commercial reverse transcriptases for tRNA sequencing library preparation

    doi: 10.1080/15476286.2026.2720028

    Figure Lengend Snippet: tRNA column and gel extraction for sequencing. (A) schematic overview of the tRNA silica-based spin column purification process. First, the total RNA sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. DA/DPH denotes deacylated and dephosphorylated total RNA and encircled roman numerals I and II correspond to eluates from column I and II, respectively. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low‑range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) binding capacity of Monarch RNA Cleanup columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. input – total RNA post-phenol/BCP extraction; DA/DPH – deacylated and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) urea-PAA analysis of eluate from column I and II. Note removal of rRNA in eluate II. Equal volumes were loaded of both column eluates. (D) column enriched and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples ( n = 48). Encircled areas correspond to column enriched tRNA (green ellipse) or gel-extracted tRNA (black ellipse). (F) correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n = 5, gel extraction n = 3). Pearson’s r = 0.9998, R 2 = 0.9995.

    Article Snippet: Bands corresponding to 5.8S and 5S rRNA are indicated. (B) binding capacity of Monarch RNA Cleanup columns (10 μg, NEB) loaded with 10, 5, or 2.5 μg total RNA.

    Techniques: Gel Extraction, Sequencing, Purification, RNA Binding Assay, Marker, Binding Assay, Extraction, Analysis, Liquid Chromatography with Mass Spectroscopy

    Comparison of tRNA enrichment efficiency by extraction method.

    Journal: RNA Biology

    Article Title: Production of MarathonRT and its comparison with commercial reverse transcriptases for tRNA sequencing library preparation

    doi: 10.1080/15476286.2026.2720028

    Figure Lengend Snippet: Comparison of tRNA enrichment efficiency by extraction method.

    Article Snippet: Bands corresponding to 5.8S and 5S rRNA are indicated. (B) binding capacity of Monarch RNA Cleanup columns (10 μg, NEB) loaded with 10, 5, or 2.5 μg total RNA.

    Techniques: Comparison, Extraction, Concentration Assay, Gel Extraction